Empty Disposable Gravity Flow Columns, Fritted, 3 mL, 0.9 x 6 cm, with 20 µm frits

Empty Disposable Gravity Flow Columns, Fritted, 3 mL, 0.9 x 6 cm, with 20 µm fritsPolypropylene (PP) chromatography column, max. vol. 4 mL, cross sectional area 0. 64 cm2, for use in low pressure chromatography. These disposable columns are economical, versatile columns for single use purification applications using gravity flow. Simple, cost effective, readily adaptable for a wide range of gravity flow applications Made of high purity medical grade polypropylene, stable in commonly used chemicals and reagents Available in various

centrifugation procedure

one retaining ring

Endoproteinase Glu-C is suitable for peptide sequencing and mapping applications

Lanes: (1) Raw cell lysate

providing a more cost-effective option for applications where no column caps nor extra frits are needed

loss of the retentate on the filter is minimized

Material (End Cap): Polypropylene (PP)

1% sarcosyl Other additives ≤500 mM imidazoleat pH7

the amino acid sequence DYKDDDDK has been used as an affinity tag to fuse at the N-terminal

~40 μmol/mL medium  Matrix Highly cross-linked 6% agarose supplied as a 50% slurry Capacity (per mL medium)

the anti-DYKDDDDK 4FF affinity resin can be used in co-immunoprecipitation to analyze the protein complex that the target fusion protein is associated with

Samples are eluted isocratically