Human Genome-Wide CRISPRi Dual-sgRNA Library

Human Genome-Wide CRISPRi Dual-sgRNA LibraryPooled library of ~105,000 dual sgRNA constructs targeting ~19,000 human protein coding genes. Each construct expresses two sgRNA targeting the same gene. Designed to work with deactivated spCas9 (dCas9) complexed with the KRAB repressor to inactivate or down regulate gene expression. Five CRISPRi dual sgRNA constructs target each gene. Designs based on Horlbeck, et al., eLife 2016; 5: e19760 DOI: 10. 7554). Manual and Product Documentation Manual:

virus SGCTL-NT-pRSGT17

The kits contain 250 µg of lentiviral packaging plasmid mix

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Manual and Product Documentation Manual: DriverMap Targeted Expression Profiling Kits

The LentiPrep Reagent Set includes the following:

87K Human CRISPR Knockout Library (4 sgRNAs/gene) comprises ~87

The DriverMap™ AIR DNA Assay for Mouse is a streamlined

virus) SVTTAP-V Puro_virus

These polyclonal cell lines stably express HLA-A02:01

virus  1 x 10^8 TU  Order Now > BCXP10M5BfpBle-P CloneTracker XP™ 10M Barcode-5' Library in pScribe12

Lentiviral Construct Packaging

The kit contains pseudoviral packaged lentiviral constructs with a green fluorescent protein (GFP) gene that increases in expression when expressed in cells with active dCas9-TA