PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF, Prepacked Cartridge

PROTEINDEX™ HiBond™ Ni-NTA Agarose 6FF, Prepacked CartridgePROTEINDEX HiBond Ni NTA Agarose 6FF has been optimized for automated purification applications. It has been widely used in scale up and preparative purification of histidine tagged proteins that are expressed in various hosts, such as E. coli, yeast, insect cells, and mammalian cells. The base matrix of PROTEINDEX HiBond Ni NTA Agarose 6FF is highly crosslinked 6% agarose, offering excellent chemical , and mechanical stability for automated

> 10 mg MBP tagged protein (80 kDa)/mL medium

3 MPa (3 bar) Chemical compatibility Reducing agents 5 mM DTE

The binding of rProtein A/G to immunoglobulins shows less pH-dependent than Protein A

NaCl (>200 mM)

thermal performance

ProteoSure™ Modified Recombinant Trypsin showed a peak at the same position before and after 3 hour incubation at 37°C

cross-sectional area 0

Isopropyl Alcohol

Polyethylene (frits)

With high sample-loading capacity and ready-to-use design

Ligand Nickel Binding capacity >40 mg 6xHis-tagged protein/mL medium Matrix 6% highly cross-linked agarose Particle size 45 μm - 165 μm Column volume 1 mL or 5 mL Maximum pressure

Ligand Nickel Binding capacity >40 mg 6xHis-tagged protein/mL medium Matrix 6% highly cross-linked agarose supplied as 50% slurry Particle size 45 μm - 165 μm Maximum pressure