Lentivirus Expressing human ACE2 Gene with BlastR

Lentivirus Expressing human ACE2 Gene with BlastRLentiviral constructs expressing the ACE2 cell receptor which mediates binding and entry by the Spike protein expressed by the SARS virus. This ready made construct can be used to make ACE2 expressing mammalian cell lines susceptible to infection by SARS CoV, SARS CoV 2 (COVID 19), viruses pseudotyped with the SARS spike protein. This lentiviral vector is available as plasmid and pre packaged virus Catalog # Description Vector RSVACE2 P ACE2 Gene with

Catalog #  Description   Vector SVCRU616N-L UbiC_RFP_Neo pRSG16N-U6-sg-UbiC-RFP-2A-Neo SVCRU616-L UbiC_RFP_Puro pRSG16-U6-sg-UbiC-RFP-2A-Puro SVCRU617-L UbiC_GFP_Puro pRSG17-U6-sg-UbiC-GFP-2A-Puro SVCRU617H-L UbiC_GFP_Hygro pRSG17H-U6-sg-UbiC-GFP-2A-Hygro SVCRU620-L CMV_RFP_Puro pRSG20-U6-sg-CMV-RFP-2A-Puro SVCRU620B-L CMV_RFP_Blast pRSG20Bla-U6-sg-CMV-RFP-2A-Blast SVCRU621-L CMV_GFP_Puro pRSG21-U6-sg-CMV-GFP-2A-Puro SVCRU6UP-L UbiC__Puro pRSGUP-U6-sg-UbiC-Puro SVCRU6CP-L CMV__Puro pRSGCP-U6-sg-CMV-Puro SVCRU6EP-L EF1__Puro pRSGEP-U6-sg-EF1-Puro SVCRU6EGP-L EF1_GFP_Puro pRSGEGP-U6-sg-EF1-GFP-2A-Puro SVCRU6ERH-L EF1_RFP_Hygro pRSGERH-U6-sg-EF1-TagRFP-2A-Hygro Manual and Product Documentation Manuals:

The SARS Spike Protein Pseudotyped Lentiviral Packaging Mix uses the same packaging system

a compatible Universal Unique Dual Index (UDI) NGS Index Plate must be purchased separately as a required component of the workflow

and reagents required for library preparation

Manual: CRISPR Pooled Lentiviral sgRNA Libraries Manual

tumorigenesis

Cross-Contamination Detection:  Triplex Isoform Pools can measure cross-contamination between different samples

Non-Targeting CRISPR control in pRSGT17-U6Tet-sg-CMV-TetR-GFP-Puro Vector

Package any third-generation lentviral vector as SARS-CoV spike protein (S-protein) pseudotyped lentiviral particles that can be used to run coronavirus neutralization assays and similar infection studies under BSL-2 guidelines

Module 1 of Cellecta's Human Genome-Wide (hGW) shRNA Library

but can be used to knockdown luciferase in cells engineered to express the targeted luciferase variation

Express and transduce any TCR sequence using Cellecta's Antigen Cloning Vectors (coming soon)