Nickel NTA Agarose Beads

Nickel NTA Agarose BeadsGoldBios Nickel NTA Agarose Beads are affinity chromatography resins for the purification of polyhistidine tagged recombinant proteins under native or denaturing conditions. The resin consists of nickel ions chelated to nitrilotriacetic acid (NTA) groups immobilized on agarose beads, enabling selective binding of histidine rich protein sequences through metal coordination interactions. Nickel NTA purification systems are among the most widely used

Mechanism of Action & Biological Relevance

Use a reference wavelength above 600 nm if available

Spectinomycin is an aminocyclitol antibiotic isolated from Streptomyces spectabilis

and a luciferase reporter gene

DL-Methionine sulfoxide the product of oxidation of the sulfur the naturally occurring amino acid methionine

In the presence of 10mM cysteine

Low phytotoxicity vs

5-dimethoxy-2- nitrophenyl) ethyl ester

a range reported as non-toxic to protoplasts under standard culture conditions

It has been used to protect against rotenone-induced apoptosis in neuroblastoma cells

N-Acetyl-DL-methionine

Pre-Weighed for Accuracy and Convenience – Eliminates measurement errors