CRISPRtest™ Essential-Gene Cas9 Knockout Assay Human or Mouse Cells (for 5 cell line pairs)

CRISPRtest™ Essential-Gene Cas9 Knockout Assay Human or Mouse Cells (for 5 cell line pairs)CRISPRtest Essential Gene Cas9 Activity Kit for Human or Mouse cells is an easy to use, highly sensitive kit for measuring functional Cas9 activity in human or mouse cell lines. The kit contains two pseudoviral packaged, lentiviral constructs one (1) that expresses an sgRNA that targets an essential gene and one fluorescent protein (e. g., GFP, BFP) as well as a second construct with a non target sgRNA and a second fluorescent marker (usually RFP or

compressed): hGW 55K Module 1

and each gene is targeted by 8 hairpins

and reagents required for target enrichment and library preparation

400 intergenic sgRNAs

each contains common

Video: Improved Adaptive Immune Receptor Repertoire Profiling for Biomarker Discovery (23:08)

Gene List: Human Genome-Wide CRISPRa sgRNA Library Gene List

7 (20I/501Y

Vector that expresses the Cas9 protein and is designed for cloning and expression of sgRNA

where you would send us your flow-sorted cells on a 96-well plate(s)

Hybrid Human-Mouse) - V2 (Online or PDF)

NextSeq 2000