Human Genome-Wide CRISPRa sgRNA Library

Human Genome-Wide CRISPRa sgRNA LibraryPooled sgRNA Library designed to work with deactivated spCas9 (dCas9) complexed with an activator (e. g., dCas9 VPH) to activate gene expression. Five sgRNA to each gene target. Library includes 3,700 non targeting sgRNA controls. Designs based on Horlbeck, et al., eLife 2016; 5: e19760 DOI: 10. 7554). Manual and Product Documentation Manual: CRISPR Pooled Lentiviral sgRNA Libraries Gene List: Human Genome Wide CRISPRa sgRNA Library Gene List Flyer:

Parallel runs of DriverMap AIR DNA Assay with DriverMap AIR RNA Assay reveal potential disease-associated

and mouse/human hybrid T-cell (TRA-TRB) or B-cell (IGH-IGK-IGL) receptors

shRNA targeting the luciferase protein in a standard lentiviral construct

IGK and IGL chains are shown

UbiC-Puro (pRSGUPS2-U6-sg-UbiC-Puro vector)

each contains common

compressed): hGW 55K Module 3 shRNA Library

such as PBMC

dividing or non-dividing cells

We have designed primers 80-200 nt in length to target the conserved regions of all human protein-coding genes and optimized them for reliable results in an efficient

After transduction and antibiotic selection of cells

Five CRISPRa dual-sgRNA constructs target each gene